OrangerV1, Main, Exploration, bibRecord, 000C53

Isolation and Characterization of Putative Endophytic Bacteria Antagonistic to Phoma tracheiphila and Verticillium albo-atrum

Identifieur interne : 000C53 ( Main/Exploration ); précédent : 000C52; suivant : 000C54

Isolation and Characterization of Putative Endophytic Bacteria Antagonistic to Phoma tracheiphila and Verticillium albo-atrum

Auteurs : Leila Kalai-Grami [Tunisie] ; Sabrine Saidi [Tunisie] ; Sarra Bachkouel [Tunisie] ; IMEN BEN SLIMENE [Tunisie] ; Monia Mnari-Hattab [Tunisie] ; MOHAMED REBAH HAJLAOUI [Tunisie] ; Ferid Limam [Tunisie]

Source :

Applied biochemistry and biotechnology [ 0273-2289 ] ; 2014.

RBID : Pascal:14-0247586

Descripteurs français

Pascal (Inist)
- Isolement, Caractérisation, Endophyte, Bactérie, Antagoniste, Citrus, Medicago truncatula, Phoma tracheiphila, Verticillium albo-atrum, Bacillus, Diversité génétique.

English descriptors

KwdEn :
- Antagonist, Bacillus, Bacteria, Characterization, Citrus, Endophyte, Genetic diversity, Isolation, Medicago truncatula, Phoma tracheiphila, Verticillium albo-atrum.

Abstract

A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.

Affiliations:

Tunisie

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antagonist</term>
<term>Bacillus</term>
<term>Bacteria</term>
<term>Characterization</term>
<term>Citrus</term>
<term>Endophyte</term>
<term>Genetic diversity</term>
<term>Isolation</term>
<term>Medicago truncatula</term>
<term>Phoma tracheiphila</term>
<term>Verticillium albo-atrum</term>
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<term>Caractérisation</term>
<term>Endophyte</term>
<term>Bactérie</term>
<term>Antagoniste</term>
<term>Citrus</term>
<term>Medicago truncatula</term>
<term>Phoma tracheiphila</term>
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<front><div type="abstract" xml:lang="en">A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.</div>
</front>
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<name sortKey="Limam, Ferid" sort="Limam, Ferid" uniqKey="Limam F" first="Ferid" last="Limam">Ferid Limam</name>
<name sortKey="Mnari Hattab, Monia" sort="Mnari Hattab, Monia" uniqKey="Mnari Hattab M" first="Monia" last="Mnari-Hattab">Monia Mnari-Hattab</name>
<name sortKey="Mohamed Rebah Hajlaoui" sort="Mohamed Rebah Hajlaoui" uniqKey="Mohamed Rebah Hajlaoui" last="Mohamed Rebah Hajlaoui">MOHAMED REBAH HAJLAOUI</name>
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Isolation and Characterization of Putative Endophytic Bacteria Antagonistic to Phoma tracheiphila and Verticillium albo-atrum

Isolation and Characterization of Putative Endophytic Bacteria Antagonistic to Phoma tracheiphila and Verticillium albo-atrum

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri